human blca cells t24 Search Results


human blca cell lines  (ATCC)
98
ATCC human blca cell lines
TRIM38 inhibition enhanced <t>BLCA</t> proliferation, migration and stemness features. a Western blot assay exhibiting the knockout efficiency of TRIM38 <t>in</t> <t>T24</t> and EJ cells. b Western blot assay showing the overexpression of TRIM38 in T24 and EJ cells. c Colony formation capacity was determined in Ctrl and TRIM38-KO cells (T24 and EJ). Quantification of results were showed on the right panel. d CCK-8 assays were conducted to exhibit the cell growth in the indicated cells (T24, EJ, 5637) at every timepoints. e Transwell assays showed that the invasive capacity of BLCA cells was enhanced with TRIM38 ablation (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. f The invasive ability of EJ cells was suppressed when cells were transfected with TRIM38 (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. g Sphere formation assay was conducted to confirm that TRIM38 deficiency could notably drive stemness features of T24 cells. Quantification of results was exhibited on the right panel. Scale bars = 200 μm
Human Blca Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mb49  (CancerTools Org)
99
CancerTools Org mb49
TRIM38 inhibition enhanced <t>BLCA</t> proliferation, migration and stemness features. a Western blot assay exhibiting the knockout efficiency of TRIM38 <t>in</t> <t>T24</t> and EJ cells. b Western blot assay showing the overexpression of TRIM38 in T24 and EJ cells. c Colony formation capacity was determined in Ctrl and TRIM38-KO cells (T24 and EJ). Quantification of results were showed on the right panel. d CCK-8 assays were conducted to exhibit the cell growth in the indicated cells (T24, EJ, 5637) at every timepoints. e Transwell assays showed that the invasive capacity of BLCA cells was enhanced with TRIM38 ablation (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. f The invasive ability of EJ cells was suppressed when cells were transfected with TRIM38 (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. g Sphere formation assay was conducted to confirm that TRIM38 deficiency could notably drive stemness features of T24 cells. Quantification of results was exhibited on the right panel. Scale bars = 200 μm
Mb49, supplied by CancerTools Org, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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resource source identifier human blca cell line rt4  (ATCC)
96
ATCC resource source identifier human blca cell line rt4
TRIM38 inhibition enhanced <t>BLCA</t> proliferation, migration and stemness features. a Western blot assay exhibiting the knockout efficiency of TRIM38 <t>in</t> <t>T24</t> and EJ cells. b Western blot assay showing the overexpression of TRIM38 in T24 and EJ cells. c Colony formation capacity was determined in Ctrl and TRIM38-KO cells (T24 and EJ). Quantification of results were showed on the right panel. d CCK-8 assays were conducted to exhibit the cell growth in the indicated cells (T24, EJ, 5637) at every timepoints. e Transwell assays showed that the invasive capacity of BLCA cells was enhanced with TRIM38 ablation (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. f The invasive ability of EJ cells was suppressed when cells were transfected with TRIM38 (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. g Sphere formation assay was conducted to confirm that TRIM38 deficiency could notably drive stemness features of T24 cells. Quantification of results was exhibited on the right panel. Scale bars = 200 μm
Resource Source Identifier Human Blca Cell Line Rt4, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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blca cell lines  (ATCC)
97
ATCC blca cell lines
TRIM38 inhibition enhanced <t>BLCA</t> proliferation, migration and stemness features. a Western blot assay exhibiting the knockout efficiency of TRIM38 <t>in</t> <t>T24</t> and EJ cells. b Western blot assay showing the overexpression of TRIM38 in T24 and EJ cells. c Colony formation capacity was determined in Ctrl and TRIM38-KO cells (T24 and EJ). Quantification of results were showed on the right panel. d CCK-8 assays were conducted to exhibit the cell growth in the indicated cells (T24, EJ, 5637) at every timepoints. e Transwell assays showed that the invasive capacity of BLCA cells was enhanced with TRIM38 ablation (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. f The invasive ability of EJ cells was suppressed when cells were transfected with TRIM38 (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. g Sphere formation assay was conducted to confirm that TRIM38 deficiency could notably drive stemness features of T24 cells. Quantification of results was exhibited on the right panel. Scale bars = 200 μm
Blca Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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um-uc-14  (CancerTools Org)
99
CancerTools Org um-uc-14
TRIM38 inhibition enhanced <t>BLCA</t> proliferation, migration and stemness features. a Western blot assay exhibiting the knockout efficiency of TRIM38 <t>in</t> <t>T24</t> and EJ cells. b Western blot assay showing the overexpression of TRIM38 in T24 and EJ cells. c Colony formation capacity was determined in Ctrl and TRIM38-KO cells (T24 and EJ). Quantification of results were showed on the right panel. d CCK-8 assays were conducted to exhibit the cell growth in the indicated cells (T24, EJ, 5637) at every timepoints. e Transwell assays showed that the invasive capacity of BLCA cells was enhanced with TRIM38 ablation (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. f The invasive ability of EJ cells was suppressed when cells were transfected with TRIM38 (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. g Sphere formation assay was conducted to confirm that TRIM38 deficiency could notably drive stemness features of T24 cells. Quantification of results was exhibited on the right panel. Scale bars = 200 μm
Um Uc 14, supplied by CancerTools Org, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mem corning 10-009-cv  (Corning Life Sciences)
90
Corning Life Sciences mem corning 10-009-cv
TRIM38 inhibition enhanced <t>BLCA</t> proliferation, migration and stemness features. a Western blot assay exhibiting the knockout efficiency of TRIM38 <t>in</t> <t>T24</t> and EJ cells. b Western blot assay showing the overexpression of TRIM38 in T24 and EJ cells. c Colony formation capacity was determined in Ctrl and TRIM38-KO cells (T24 and EJ). Quantification of results were showed on the right panel. d CCK-8 assays were conducted to exhibit the cell growth in the indicated cells (T24, EJ, 5637) at every timepoints. e Transwell assays showed that the invasive capacity of BLCA cells was enhanced with TRIM38 ablation (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. f The invasive ability of EJ cells was suppressed when cells were transfected with TRIM38 (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. g Sphere formation assay was conducted to confirm that TRIM38 deficiency could notably drive stemness features of T24 cells. Quantification of results was exhibited on the right panel. Scale bars = 200 μm
Mem Corning 10 009 Cv, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rpmi 1640 medium corning 10-040-cv  (Corning Life Sciences)
90
Corning Life Sciences rpmi 1640 medium corning 10-040-cv
TRIM38 inhibition enhanced <t>BLCA</t> proliferation, migration and stemness features. a Western blot assay exhibiting the knockout efficiency of TRIM38 <t>in</t> <t>T24</t> and EJ cells. b Western blot assay showing the overexpression of TRIM38 in T24 and EJ cells. c Colony formation capacity was determined in Ctrl and TRIM38-KO cells (T24 and EJ). Quantification of results were showed on the right panel. d CCK-8 assays were conducted to exhibit the cell growth in the indicated cells (T24, EJ, 5637) at every timepoints. e Transwell assays showed that the invasive capacity of BLCA cells was enhanced with TRIM38 ablation (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. f The invasive ability of EJ cells was suppressed when cells were transfected with TRIM38 (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. g Sphere formation assay was conducted to confirm that TRIM38 deficiency could notably drive stemness features of T24 cells. Quantification of results was exhibited on the right panel. Scale bars = 200 μm
Rpmi 1640 Medium Corning 10 040 Cv, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cupri-tartaric solution 1  (LGC Standards)
99
LGC Standards cupri-tartaric solution 1
TRIM38 inhibition enhanced <t>BLCA</t> proliferation, migration and stemness features. a Western blot assay exhibiting the knockout efficiency of TRIM38 <t>in</t> <t>T24</t> and EJ cells. b Western blot assay showing the overexpression of TRIM38 in T24 and EJ cells. c Colony formation capacity was determined in Ctrl and TRIM38-KO cells (T24 and EJ). Quantification of results were showed on the right panel. d CCK-8 assays were conducted to exhibit the cell growth in the indicated cells (T24, EJ, 5637) at every timepoints. e Transwell assays showed that the invasive capacity of BLCA cells was enhanced with TRIM38 ablation (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. f The invasive ability of EJ cells was suppressed when cells were transfected with TRIM38 (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. g Sphere formation assay was conducted to confirm that TRIM38 deficiency could notably drive stemness features of T24 cells. Quantification of results was exhibited on the right panel. Scale bars = 200 μm
Cupri Tartaric Solution 1, supplied by LGC Standards, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ihc  (Human Protein Atlas)
86
Human Protein Atlas ihc
TRIM38 inhibition enhanced <t>BLCA</t> proliferation, migration and stemness features. a Western blot assay exhibiting the knockout efficiency of TRIM38 <t>in</t> <t>T24</t> and EJ cells. b Western blot assay showing the overexpression of TRIM38 in T24 and EJ cells. c Colony formation capacity was determined in Ctrl and TRIM38-KO cells (T24 and EJ). Quantification of results were showed on the right panel. d CCK-8 assays were conducted to exhibit the cell growth in the indicated cells (T24, EJ, 5637) at every timepoints. e Transwell assays showed that the invasive capacity of BLCA cells was enhanced with TRIM38 ablation (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. f The invasive ability of EJ cells was suppressed when cells were transfected with TRIM38 (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. g Sphere formation assay was conducted to confirm that TRIM38 deficiency could notably drive stemness features of T24 cells. Quantification of results was exhibited on the right panel. Scale bars = 200 μm
Ihc, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rpmi 1640  (GE Healthcare)
94
GE Healthcare rpmi 1640
TRIM38 inhibition enhanced <t>BLCA</t> proliferation, migration and stemness features. a Western blot assay exhibiting the knockout efficiency of TRIM38 <t>in</t> <t>T24</t> and EJ cells. b Western blot assay showing the overexpression of TRIM38 in T24 and EJ cells. c Colony formation capacity was determined in Ctrl and TRIM38-KO cells (T24 and EJ). Quantification of results were showed on the right panel. d CCK-8 assays were conducted to exhibit the cell growth in the indicated cells (T24, EJ, 5637) at every timepoints. e Transwell assays showed that the invasive capacity of BLCA cells was enhanced with TRIM38 ablation (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. f The invasive ability of EJ cells was suppressed when cells were transfected with TRIM38 (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. g Sphere formation assay was conducted to confirm that TRIM38 deficiency could notably drive stemness features of T24 cells. Quantification of results was exhibited on the right panel. Scale bars = 200 μm
Rpmi 1640, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


TRIM38 inhibition enhanced BLCA proliferation, migration and stemness features. a Western blot assay exhibiting the knockout efficiency of TRIM38 in T24 and EJ cells. b Western blot assay showing the overexpression of TRIM38 in T24 and EJ cells. c Colony formation capacity was determined in Ctrl and TRIM38-KO cells (T24 and EJ). Quantification of results were showed on the right panel. d CCK-8 assays were conducted to exhibit the cell growth in the indicated cells (T24, EJ, 5637) at every timepoints. e Transwell assays showed that the invasive capacity of BLCA cells was enhanced with TRIM38 ablation (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. f The invasive ability of EJ cells was suppressed when cells were transfected with TRIM38 (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. g Sphere formation assay was conducted to confirm that TRIM38 deficiency could notably drive stemness features of T24 cells. Quantification of results was exhibited on the right panel. Scale bars = 200 μm

Journal: Journal of Translational Medicine

Article Title: TRIM38 triggers the uniquitination and degradation of glucose transporter type 1 (GLUT1) to restrict tumor progression in bladder cancer

doi: 10.1186/s12967-021-03173-x

Figure Lengend Snippet: TRIM38 inhibition enhanced BLCA proliferation, migration and stemness features. a Western blot assay exhibiting the knockout efficiency of TRIM38 in T24 and EJ cells. b Western blot assay showing the overexpression of TRIM38 in T24 and EJ cells. c Colony formation capacity was determined in Ctrl and TRIM38-KO cells (T24 and EJ). Quantification of results were showed on the right panel. d CCK-8 assays were conducted to exhibit the cell growth in the indicated cells (T24, EJ, 5637) at every timepoints. e Transwell assays showed that the invasive capacity of BLCA cells was enhanced with TRIM38 ablation (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. f The invasive ability of EJ cells was suppressed when cells were transfected with TRIM38 (left panel). Quantification of results was exhibited on the right panel. Scale bars = 250 μm. g Sphere formation assay was conducted to confirm that TRIM38 deficiency could notably drive stemness features of T24 cells. Quantification of results was exhibited on the right panel. Scale bars = 200 μm

Article Snippet: The human BLCA cell lines (T24, EJ and 5637) and 293 T cells were obtained from the American Type Culture Collection (ATCC).

Techniques: Inhibition, Migration, Western Blot, Knock-Out, Over Expression, CCK-8 Assay, Transfection, Tube Formation Assay

GLUT1 inhibitor (BAY-876) was effective to inhibit the progression of TRIM38 low/− bladder cancer. a The half maximal inhibitory concentration (IC50) values of BAY-876 (GLUT1 inhibitor) in BLCA cell lines (T24, EJ and 5637). b – d BAY-876 could suppress the cell growth of BLCA cells in a dose-dependent manner. e Representative image inhibited that BAY-876 could inhibit the tumor volumes derived from TRIM38-KO cells relative to tumors derived from control cells. f The tumor volumes were detected and recorded at the indicated timepoints and the growth curve was generated. g Tumors were resected and weighed to compare the differences in the indicated groups

Journal: Journal of Translational Medicine

Article Title: TRIM38 triggers the uniquitination and degradation of glucose transporter type 1 (GLUT1) to restrict tumor progression in bladder cancer

doi: 10.1186/s12967-021-03173-x

Figure Lengend Snippet: GLUT1 inhibitor (BAY-876) was effective to inhibit the progression of TRIM38 low/− bladder cancer. a The half maximal inhibitory concentration (IC50) values of BAY-876 (GLUT1 inhibitor) in BLCA cell lines (T24, EJ and 5637). b – d BAY-876 could suppress the cell growth of BLCA cells in a dose-dependent manner. e Representative image inhibited that BAY-876 could inhibit the tumor volumes derived from TRIM38-KO cells relative to tumors derived from control cells. f The tumor volumes were detected and recorded at the indicated timepoints and the growth curve was generated. g Tumors were resected and weighed to compare the differences in the indicated groups

Article Snippet: The human BLCA cell lines (T24, EJ and 5637) and 293 T cells were obtained from the American Type Culture Collection (ATCC).

Techniques: Concentration Assay, Derivative Assay, Control, Generated